Comprehensive Evaluation of Doxorubicin-Loaded Moronic Acid Nanoparticles: A Multidimensional Study on Antioxidant, Anti-inflammatory, and Anticancer Efficacy

AUTHOR

Rajni Singh*, Devesh Mishra Department of Botany, OSGU, Hisar

ABSTRACT  

In this study, we explored the therapeutic potential of Doxorubicin-loaded Moronic Acid Nanoparticles (Dox-MA NPs) by evaluating their antioxidant, anti-inflammatory, and anticancer properties. Doxorubicin, a potent chemotherapeutic agent, is known for its systemic toxicity, including cardiotoxicity, which limits its clinical application. By encapsulating doxorubicin within Moronic acid-based nanoparticles, we aimed to provide controlled drug release, thereby reducing toxic side effects and improving therapeutic outcomes. The nanoparticles were synthesized using a solvent evaporation method and characterized for their size, zeta potential, and encapsulation efficiency. Antioxidant activity was assessed using the DPPH radical scavenging assay, anti-inflammatory activity was evaluated by the hemoglobin method, and anticancer activity was tested using MTT cell viability assays in MCF-7 and HeLa cancer cell lines. The results indicated a dose-dependent increase in antioxidant and antiinflammatory activities, with Dox-MA NPs demonstrating significant cytotoxicity in both cancer cell lines. These findings suggest that Dox-MA NPs have promising therapeutic potential in reducing oxidative stress, inflammation, and cancer cell viability. Further optimization of the formulation is needed to enhance their consistency and efficacy for clinical applications.

Keywords: Antioxidant Activity, Anti-inflammatory Activity, Anticancer Activity, Doxorubicin, Moronic Acid Nanoparticles