Engineered MgO–Doxycycline Nanocomposites for Improved Antibacterial Performance
Abstract
The increasing prevalence of multidrug-resistant Salmonella Typhi poses a significant threat to public health, necessitating novel therapeutic approaches. This study investigates magnesium oxide (MgO) nanoparticles synthesized in combination with doxycycline to enhance antimicrobial efficacy. MgO- doxycycline nanoparticles were synthesized via a co-precipitation method, optimized for particle size, stability, and drug loading efficiency. Characterization studies confirmed successful nanoparticle formation with an average particle size of 72.5 nm, a zeta potential of +28.4 mV, and a polydispersity index (PDI) of 0.221, indicating uniform particle distribution. Fourier-transform infrared spectroscopy (FTIR) revealed characteristic peaks at 460 cm⁻¹ (Mg-O stretching) and 1642 cm⁻¹ (doxycycline carbonyl group), confirming successful drug incorporation. X-ray diffraction (XRD) analysis indicated strong diffraction peaks at 2θ = 36.8°, 42.9°, and 62.3°, signifying high crystallinity. Scanning electron microscopy (SEM) exhibited spherical morphology with well-defined surfaces. Drug release studies in simulated intestinal fluid demonstrated sustained release, with 82.3% of doxycycline released over 24 hours. In vitro antimicrobial assays against multidrug-resistant Salmonella Typhi revealed a significant reduction in bacterial growth, with a minimum inhibitory concentration (MIC) of 4 µg/mL. The MgO- doxycycline nanoparticle system thus demonstrates potential as an effective therapeutic agent for combating antibiotic-resistant Salmonella Typhi, warranting further investigation in vivo.
How to Cite
Anju, Sandeep Kumar, "Engineered MgO–Doxycycline Nanocomposites for Improved Antibacterial Performance", Vol. 3, Issue 1, 12-04-2025, pp. 50-65.
