Anti-inflammatory and Antioxidant Evaluation of Tamoxifen-Loaded Moronic Acid Nanoparticles: A Multifunctional Approach for Cancer Therapeutics

AUTHOR

Rajni Singh, Devesh Mishra
Department of Botany, OSGU, Hisar

ABSTRACT  

This study investigates the anti-inflammatory and antioxidant activities of tamoxifen-loaded moronic acid nanoparticles (TAM-MA NPs), a novel nanoformulation previously developed for enhanced anticancer efficacy. TAM-MA NPs were synthesized using a solvent evaporation method and characterized for size, zeta potential, and drug loading efficiency. In-vitro assays revealed a significant, concentration-dependent inhibition of protein denaturation and erythrocyte membrane lysis, indicating strong anti-inflammatory potential. Additionally, TAMMA NPs demonstrated potent antioxidant activity across DPPH, hydrogen peroxide scavenging, and total antioxidant capacity (TAC) assays. At the highest concentration tested (100 µg/mL), the formulation achieved 86.3% protein denaturation inhibition, 82.7% membrane stabilization, and 186.4 µg AAE/mg TAC. Statistical analysis confirmed the significance of all findings (p < 0.0001). These results suggest that TAM-MA NPs offer multifunctional therapeutic potential by simultaneously addressing oxidative stress and inflammation, thereby enhancing their value in cancer management

Keywords: Anti-inflammatory activity, Antioxidant capacity, Cancer nanotherapy, Moronic acid, Nanoparticles, Tamoxifen